Open AccessAntimicrobial susceptibility of Mycoplasma genitalium isolates from Pretoria, South Africa in 2012 and 2016
Author(s) -
M Roux,
Maanda Mafunise,
Barbara E De Villiers,
R.M.M. Ditsele
Publication year - 2018
Publication title -
southern african journal of infectious diseases
Language(s) - English
Resource type - Journals
eISSN - 2313-1810
pISSN - 2312-0053
DOI - 10.4102/sajid.v33i2.17
Subject(s) - mycoplasma genitalium , azithromycin , cervicitis , urethritis , microbiology and biotechnology , 23s ribosomal rna , virology , genotype , antibiotic resistance , medicine , biology , chlamydia trachomatis , antibiotics , gene , gynecology , genetics , ribosome , rna
Background: Mycoplasma genitalium is a sexually transmitted pathogen associated with non-gonococcal urethritis and cervicitis. Azithromycin regimens have been considered first-line treatment for M. genitalium infections, with fluoroquinolone regimens effective as second-line treatments. However, the proportion of M. genitalium harbouring macrolide or fluoroquinolone resistance-associated mutations has been increasing worldwide. This study was done to compare the genotypic macrolide and fluoroquinolone resistance of M. genitalium strains obtained from women attending a termination of pregnancy clinic five years apart.Methods: M. genitalium was detected by PCR in vaginal swab samples from 100 and 104 termination of pregnancy attendees at a tertiary hospital in Pretoria, South Africa during 2012 and 2016 respectively. Genes associated with macrolide and fluoroquinolone resistance in the M. genitalium isolates were sequenced and analysed.Results: The prevalence of M. genitalium was 6.0% (6/100) in 2012 and 7.7% (8/104) in 2016. No resistance-associated mutations were seen in the 2012 isolates. Among the 2016 M. genitalium isolates, two (25%) harboured a macrolide-associated resistance mutation and one (12.5%) a fluoroquinolone resistance-associated mutation in the parC gene.Conclusions: There is an increase in macrolide and fluoroquinolone resistance among local M. genitalium strains. This highlights the need for improved surveillance.