Open AccessDetection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2
Author(s) -
Tuba Çiğdem Oğuzoğlu,
Seçkin Salar,
Ece Adıgüzel,
Cansu Demirden,
O. Ülgenalp
Publication year - 2020
Publication title -
onderstepoort journal of veterinary research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.563
H-Index - 35
eISSN - 2219-0635
pISSN - 0030-2465
DOI - 10.4102/ojvr.v87i1.1886
Subject(s) - amplicon , biology , viral tegument , polymerase chain reaction , gene , virology , herd , dna sequencing , single nucleotide polymorphism , base pair , dna , genetics , genotype , zoology
In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited.