Open AccessA rapid and sensitive real-time reverse transcription PCR for the pathotyping of South African H5N2 avian influenza viruses : research communication
Author(s) -
Célia Abolnik
Publication year - 2008
Publication title -
onderstepoort journal of veterinary research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.563
H-Index - 35
eISSN - 2219-0635
pISSN - 0030-2465
DOI - 10.4102/ojvr.v75i4.110
Subject(s) - influenza a virus subtype h5n1 , virology , outbreak , reverse transcriptase , reverse transcription polymerase chain reaction , biology , highly pathogenic , virus , microbiology and biotechnology , gene , polymerase chain reaction , genetics , messenger rna
A Fluorescence resonance energy transfer (FRET) real-time reverse-transcription (rRT-PCR) assay was developed that distinguishes stains of South African and European highly pathogenic (HPAI) from low pathogenicity (LPAI) H5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (H0). The assay was used to pathotype H5-type viruses detected by rRT-PCR in ostrich tracheal swabs collected during the 2006 HPAI H5N2 outbreak in the Western Cape Province.