Open AccessAnticarcinogenic effect of alpha-lipoic acid and <i>Jatropha gossypifolia</i> leaves against cypermethrin-induced genotoxicity in rats
Author(s) -
Opeyemi Faokunla,
Oluyemi Akinloye,
Regigozi Ugbaja,
Abideen Idowu Adeogun
Publication year - 2018
Publication title -
journal of medicinal plants for economic development
Language(s) - English
Resource type - Journals
eISSN - 2616-4809
pISSN - 2519-559X
DOI - 10.4102/jomped.v2i1.45
Subject(s) - genotoxicity , micronucleus test , pharmacology , chemistry , traditional medicine , pyrethroid , micronucleus , biology , toxicity , medicine , pesticide , organic chemistry , agronomy
Background: Cypermethrin (Cyp), a synthetic pyrethroid developed for agricultural and public health applications, has become harmful to the environment and mammalian life owing to indiscriminate use.Aim: This study aimed to investigate the protective effect of the methanolic extract of Jatropha gossypifolia leaves against Cyp-induced genotoxicity in rats using alpha-lipoic acid (α-LA) as standard drug.Method: Fifty-six male Wistar rats, assigned to eight groups of seven animals each, were treated orally for 28 days as follows - Group 1: only corn oil (Control); Group 2: 20 mg/kg body weight (b.wt) of Cyp; Groups 3, 4 and 5: Cyp + 50 mg/kg b.wt of J. gossypifolia, Cyp + 100 mg/kg b.wt of J. gossypifolia and Cyp + 50 mg/kg b.wt of α-LA, respectively; Groups 6, 7 and 8: J. gossypifolia (50 mg/kg b.wt), J. gossypifolia (100 mg/kg b.wt) and α-LA (50 mg/kg b.wt), respectively. The P53 was assayed for using the plasma, while the micronucleus assay was carried out on the bone marrow.Results: Results showed that exposure to Cyp significantly increased the number of micronucleated polychromatic erythrocytes (86.70%) as well as p53 concentration in both the liver and plasma (200.00% and 125.53%) compared to the control. Co-administration with methanolic extract of J. gossypifolia leaves significantly reversed these alterations in a dose-dependent manner.Conclusion: The methanolic extract of J. gossypifolia leaves has the potential to ameliorate Cyp-induced genotoxicity in rats, and also, the toxicological evaluations of the plant extract and α-LA showed that it is relatively safe for use at the doses investigated.