Open AccessGeneration of Insulin-Expressing Cells in Mouse Small Intestine by Pdx1, MafA, and BETA2/NeuroD
Author(s) -
So Hyun Lee,
Marie Rhee,
Ji Won Kim,
Kun Ho Yoon
Publication year - 2017
Publication title -
diabetes and metabolism journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.234
H-Index - 46
eISSN - 2233-6087
pISSN - 2233-6079
DOI - 10.4093/dmj.2017.41.5.405
Subject(s) - pdx1 , neurod , small intestine , progenitor cell , insulin , biology , pancreas , stem cell , medicine , endocrinology , cellular differentiation , microbiology and biotechnology , gene , biochemistry , islet
To develop surrogate insulin-producing cells for diabetes therapy, adult stem cells have been identified in various tissues and studied for their conversion into β-cells. Pancreatic progenitor cells are derived from the endodermal epithelium and formed in a manner similar to gut progenitor cells. Here, we generated insulin-producing cells from the intestinal epithelial cells that induced many of the specific pancreatic transcription factors using adenoviral vectors carrying three genes: PMB (pancreatic and duodenal homeobox 1 [Pdx1], V-maf musculoaponeurotic fibrosarcoma oncogene homolog A [MafA], and BETA2/NeuroD).