Open AccessFECAL MICROBIOTA TRANSPLANTATION: FAST PARASITES DIAGNOSIS BY MULTIPLEX PCR
Author(s) -
Omar Mura,
Elisa Gamalero,
Luigi Di Matteo,
Andrea Rocchetti
Publication year - 2021
Publication title -
working paper of public health
Language(s) - English
Resource type - Journals
ISSN - 2279-9761
DOI - 10.4081/wpph.2021.9311
Subject(s) - feces , gold standard (test) , multiplex , biology , fecal bacteriotherapy , transplantation , multiplex polymerase chain reaction , microbiology and biotechnology , polymerase chain reaction , medicine , bioinformatics , antibiotics , clostridium difficile , biochemistry , gene
Objectives: Evaluation of the analytical performance of the Novodiag Stool Parasites biomolecular assay for the detection of intestinal parasites in faeces of the faecal microbiota transplant donor. Methodology: Comparison between the gold standard for the diagnosis of intestinal parasites, light microscopy, and the Novodiag Stool Parasites biomolecular assay through the use of 22 fecal samples. Results: Twelve out of 22 samples were positive on microscopic examination. In order to assess the overlapping between the results obtained through parasitological examination and the molecular methodology, all samples were tested to the Novodiag Stool Parasites assay. Positive samples detected by biomolecular assay were 11 out of 22. Conclusion: The preliminary evaluation of the analytical performance of the Novodiag Stool Parasites kit can be considered promising. The Novodiag Stool Parasites kit can be effectively used as a tool in the fast diagnosis of intestinal parasitoses on the faecal microbiota transplant donor faeces.