Open AccessLA RIVOLUZIONE DELLA CRIO-MICROSCOPIA ELETTRONICA NELLO STUDIO STRUTTURALE DI PROTEINE E ACIDI NUCLEICI
Author(s) -
M. Bolognesi
Publication year - 2020
Publication title -
rendiconti. classe di scienze matematiche e naturali
Language(s) - English
Resource type - Journals
eISSN - 2384-986X
pISSN - 1974-6989
DOI - 10.4081/scie.2017.638
Subject(s) - nucleic acid , ribosome , chemistry , biochemistry , rna , gene
Observing the fine details of molecular structures (e.g. in proteins and in nucleic acids) has been a central part of Structural Biology over the past 50 years. The recent advent of single particle cryo-electron microscopy brought a revolution in this field, that previously relied on X-ray crystallography and nuclear magnetic resonance. It is now possible to explore the structures of large subcellular assemblies, such as the ribosome, resolving details on the scale of amino acids and nucleotides, in favorable cases reaching the 2 Ã… resolution level.