Open AccessImpact of two different commercial DNA extraction methods on BK virus viral load
Author(s) -
Massimiliano Bergallo,
Ilaria Galliano,
E. Loiacono,
Francesca Ferro,
Paola Montanari,
Paolo Ravanini
Publication year - 2016
Publication title -
microbiologia medica
Language(s) - English
Resource type - Journals
eISSN - 2280-6423
pISSN - 1120-0146
DOI - 10.4081/mm.2016.4825
Subject(s) - taqman , extraction (chemistry) , dna extraction , polymerase chain reaction , nucleic acid , bk virus , population , viral load , real time polymerase chain reaction , virology , dna , virus , biology , chromatography , chemistry , medicine , biochemistry , genetics , environmental health , kidney transplantation , gene , kidney
Background and aim: BK virus, a member of human polyomavirus family, is a worldwide distributed virus characterized by a seroprevalence rate of 70-90% in adult population. Monitoring of viral replication is made by evaluation of BK DNA by quantitative polymerase chain reaction. Many different methods can be applied for extraction of nucleic acid from several specimens. The aim of this study was to assess the impact of two different DNA extraction procedure on BK viral load. Materials and methods: DNA extraction procedure including the Nuclisens easyMAG platform (bioMerieux, Marcy l’Etoile, France) and manual QIAGEN extraction (QIAGEN Hilden, Germany). BK DNA quantification was performed by Real Time TaqMan PCR using a commercial kit. Result and discussion: The samples capacity, cost and time spent were compared for both systems. In conclusion our results demonstrate that automated nucleic acid extraction method using Nuclisense easyMAG was superior to manual protocol (QIAGEN Blood Mini kit), for the extraction of BK virus from serum and urine specimens