Open AccessOvine fetuses from slaughterhouses: A useful source for neural cell primary cultures
Author(s) -
Vittorio Farina,
Sergio Domenico Gadau,
G Lepore,
Marcella Carcupino,
Marco Zedda
Publication year - 2021
Publication title -
journal of biological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 6
eISSN - 2284-0230
pISSN - 1826-8838
DOI - 10.4081/jbr.2021.9344
Subject(s) - fetus , biology , population , cell type , andrology , cell , cell culture , in vitro , neuroscience , anatomy , microbiology and biotechnology , pathology , pregnancy , genetics , medicine , environmental health
A lot of evidence demonstrates that sheep could represent an experimental model to set up medical procedures in view of their application on humans. Sheep are chosen as models for human biomechanical studies because their skeleton has some similarities to humans. The aim of this work was to set up sheep primary cultures from ovine fetuses at different ages, from pregnant uteri retrieved at local abattoirs. Cell characterization showed that one cell population was immunopositive to GFAP and identifiable as astrocytes, whereas a second cell type was III β-tubulin-positive, and hence classified as neurons. At 60-days old fetus is suitable to obtain neurons, whereas in a 90-days old fetus the cell culture is predominantly characterized by glial cells. The procedure here proposed is inexpensive, in fact, collecting fetuses during sheep slaughtering is a cost-saving option, unlike common experimental animals such as mice, rats, rabbits, that require very high economical efforts. Finally, our protocol fully eliminates the need of animal killing, being living animals replaced by a validated in vitro model in agreement with the 3Rs statement.