Open AccessUse of RAPD and PCR double amplification in the study of ancient DNA
Author(s) -
Francesca Balzano,
E. Mura,
Alessandra Mura,
G Lepore,
Marco Zedda
Publication year - 2011
Publication title -
journal of biological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 6
eISSN - 2284-0230
pISSN - 1826-8838
DOI - 10.4081/jbr.2011.4692
Subject(s) - rapd , amplicon , biology , mitochondrial dna , ancient dna , dna , polymerase chain reaction , genetics , primer (cosmetics) , multiple displacement amplification , gene duplication , microbiology and biotechnology , dna extraction , gene , genetic diversity , population , chemistry , demography , organic chemistry , sociology
This project analysed the DNA extracted from bones of ancient sheep which have been brought to light in Sardinian different archaeological sites. In order to better analyse this highly fragmented DNA, a double amplification technique was chosen. The first approach consisted of RAPD-PCR abd the second one in classic PCR. The RAPD-PCR amplified random fragments and allowed the production of numerous amplicons. The products of RAPD amplification have been amplified, more specifically, by the second PCR using primers for a sequence of 176 bp of mitochondrial D-loop region. These DNA fragments have been sequenced and the sequence analysis has confirmed that it belonged to Ovis aries. Consequently, this provedure can be considered a valid tool to perform amplification of degraded DNA, such as ancient DNA