Open AccessHistochemical characterization of human osteochondral tissue: comparison between healthy cartilage, arthrotic tissues, and cartilage defect treated with MACI technique
Author(s) -
Francesco Tessarolo,
R. Tatti,
Marco Molinari,
P. Dorigotti,
R. Antolini,
Giandomenico Nollo
Publication year - 2011
Publication title -
journal of biological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 6
eISSN - 2284-0230
pISSN - 1826-8838
DOI - 10.4081/jbr.2011.4688
Subject(s) - cartilage , staining , proteoglycan , pathology , matrix (chemical analysis) , articular cartilage , anatomy , extracellular matrix , biomedical engineering , chemistry , biology , medicine , microbiology and biotechnology , osteoarthritis , alternative medicine , chromatography
Matrix-induced sutologous chondrocytes implantation (MACI) is a promising technique for the treatment of articular cartilage lesions, but long time outcome have to be established. We developed and optimized specific techniques of histochemical staining to characterize healthy and pathologic osteochondral tissue. Seven different staining protocols were applied to assess tissue architecture, cells morphology, proteoglycan content, and collagen fibers distribution. Potentialities of histochemical staining and histomorphology of biopsies from second look arthroscopy will be presented