Open AccessEffects of PCB mixtures on recognized targets of endocrine disrupters exposure: search for early biomarkers
Author(s) -
Laura Stecca,
Cinzia La Rocca,
Sabrina Tait,
Alberto Mantovani
Publication year - 2011
Publication title -
journal of biological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 6
eISSN - 2284-0230
pISSN - 1826-8838
DOI - 10.4081/jbr.2011.4628
Subject(s) - endocrine system , in vitro , nuclear receptor , receptor , chemistry , biology , endocrinology , medicine , biochemistry , hormone , gene , transcription factor
Polychlorinated Biphenyls (PCBs) are Endocrine Disrupters (EDs) relevant to human dietary exposure. Since liver is the main organ involved in metabolism, a human hepatoblastoma cell line (HuH6) was used as in vitro model. A panel of nuclear receptors (NRs) was selected as early markers of PCBs exposure and analysed by qPCR. PCB congeners, grouped in three mixtures according to similarities in the modes of action, were used at the concentrations derived from previous data on human internal exposure. Preliminary results indicated that PCB mixtures exert different NRs modulations in HuH6