Open AccessCytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria
Author(s) -
Alessandro Parodi,
Anna Maria Bassi,
Mariafrancesca Catalano,
Elisabetta Giacco,
Gian Luigi Mariottini,
Luigi Pane
Publication year - 2009
Publication title -
journal of biological research
Language(s) - English
Resource type - Journals
eISSN - 2284-0230
pISSN - 1826-8838
DOI - 10.4081/jbr.2009.4734
Subject(s) - jellyfish , cnidaria , scyphozoa , aurelia aurita , biology , cnidocyte , cytotoxic t cell , nematocyst , neutral red , mtt assay , hydrozoa , microbiology and biotechnology , cytotoxicity , toxicology , biochemistry , ecology , cell , in vitro , coral
Biotoxin production is a characterizing aspect of the physiology and ecology of several marine organisms. In this study the cytotoxic effect of crude extracts from jellyfish Pelagia noctiluca, Chrysaora hysoscella and Aurelia aurita (Cnidaria: Scyphozoa) against cultured human keratinocytes NCTC 2544 was evaluated by 3- (4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Assay and Neutral Red Uptake (NRU). Low cytotoxic effects were observed with NRU, while MTT showed a dose-dependent effect, with a "plateau" at highest protein concentrations. Therefore, crude extraxts didn't show effect on lysosomes but MTT test emphasized cell survival decrease and a "plateau" effect at high extract doses which could be related with the inhibition of the mitochondrial dehydrogenase activity