Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria | Zendy

Alessandro Parodi | Zendy; A.M. Bassi | Zendy; Mariafrancesca Catalano | Zendy; Elisabetta Giacco | Zendy; G.L. Mariottini | Zendy; L. Pane | Zendy

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Cytotoxic effect on human keratinocytes of crude extracts from planktonic Cnidaria

Author(s) -

Alessandro Parodi,

A.M. Bassi,

Mariafrancesca Catalano,

Elisabetta Giacco,

G.L. Mariottini,

L. Pane

Publication year - 2009

Publication title -

journal of biological research - bollettino della società italiana di biologia sperimentale

Language(s) - English

Resource type - Journals

eISSN - 2284-0230

pISSN - 1826-8838

DOI - 10.4081/jbr.2009.4734

Subject(s) - jellyfish , cnidaria , scyphozoa , aurelia aurita , biology , cnidocyte , cytotoxic t cell , nematocyst , neutral red , mtt assay , hydrozoa , microbiology and biotechnology , cytotoxicity , toxicology , biochemistry , ecology , cell , in vitro , coral

Biotoxin production is a characterizing aspect of the physiology and ecology of several marine organisms. In this study the cytotoxic effect of crude extracts from jellyfish Pelagia noctiluca, Chrysaora hysoscella and Aurelia aurita (Cnidaria: Scyphozoa) against cultured human keratinocytes NCTC 2544 was evaluated by 3- (4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Assay and Neutral Red Uptake (NRU). Low cytotoxic effects were observed with NRU, while MTT showed a dose-dependent effect, with a "plateau" at highest protein concentrations. Therefore, crude extraxts didn't show effect on lysosomes but MTT test emphasized cell survival decrease and a "plateau" effect at high extract doses which could be related with the inhibition of the mitochondrial dehydrogenase activity

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