Open AccessIdentification and serotyping of <em>Listeria monocytogenes</em>, isolated from various salmon products, sold in retail market in Lithuania
Author(s) -
Ineta Simonavičienė,
Gintarė Zakarienė,
Aušra Lozoraitytė,
Gintarė Zaborskienė,
Gediminas Gerulis,
Artūras Stimbirys
Publication year - 2021
Publication title -
italian journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 13
ISSN - 2239-7132
DOI - 10.4081/ijfs.2021.9341
Subject(s) - listeria monocytogenes , loin , food science , serotype , listeria , fish fillet , fish <actinopterygii> , biology , microbiology and biotechnology , fishery , bacteria , genetics
Cold smoked salmon products (belly flaps, pieces, fillet, and loin) obtained from the retail market in Lithuania were tested for the presence of L. monocytogenes. It was found that contamination of the cold smoked fish products with Listeria spp. depends on the type of the product. Contamination with listeria in salmon belly flaps was 7.5 times higher than in the loin (P<0.05), 1.8 times higher than in the pieces (P<0.05) and 30 times higher than in the fillet (P<0.05). Microbiological analysis showed that 32.5% (P<0.05) of the fish product samples were infected with L. monocytogenes, while multiplex PCR confirmed 31.25% positive samples (P<0.01). According to the study results, L. monocytogenes strains were divided into two serotypes: 4b (94.6%) and 1/2a (5.4%). High contamination of the products with Listeria spp. showed that cold smoked salmon products, sold in local market, can be a reason of human listeriosis in Lithuania.