EFFECT OF SIDEROPHORES AGAINST E. COLI O157:H7 | Zendy

Filippo Giarratana | Zendy; Graziella Ziino | Zendy; D. Signorino | Zendy; Luca Giordano | Zendy; Alessandro Giuffrida | Zendy

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EFFECT OF SIDEROPHORES AGAINST E. COLI O157:H7

Author(s) -

Filippo Giarratana,

Graziella Ziino,

D. Signorino,

Luca Giordano,

Alessandro Giuffrida

Publication year - 2012

Publication title -

italian journal of food safety

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.359

H-Index - 13

ISSN - 2239-7132

DOI - 10.4081/ijfs.2012.4.57

Subject(s) - siderophore , pseudomonas fluorescens , escherichia coli , reagent , microbiology and biotechnology , chemistry , pseudomonas , escherichia , bacteria , biology , biochemistry , organic chemistry , genetics , gene

In this study, the inhibiting activity of siderophores produced by Pseudomonas fluorescens against Escherichia coli O157:7 was assayed. Siderophores were extracted from P. fluorescens cultures and preliminarily tested by C.A.S. reagent and UV. The siderophores activity against E. coli O157:H7 was characterised by the determination of Minimal Inhibiting Concentration (MIC). Furthermore, E. coli O157:H7 growth tests with and without siderophores were prepared at 12°C, pH 7 and 5, aw 0.997 and 0,988. The MIC was assessed at 7 μg/ml whereas the growth test showed a high inhibiting activity with the exception of tests at pH 5

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