Open AccessEFFECT OF SIDEROPHORES AGAINST E. COLI O157:H7
Author(s) -
Filippo Giarratana,
Graziella Ziino,
D. Signorino,
Luca Giordano,
Alessandro Giuffrida
Publication year - 2012
Publication title -
italian journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 13
ISSN - 2239-7132
DOI - 10.4081/ijfs.2012.4.57
Subject(s) - siderophore , pseudomonas fluorescens , escherichia coli , reagent , microbiology and biotechnology , chemistry , pseudomonas , escherichia , bacteria , biology , biochemistry , organic chemistry , genetics , gene
In this study, the inhibiting activity of siderophores produced by Pseudomonas fluorescens against Escherichia coli O157:7 was assayed. Siderophores were extracted from P. fluorescens cultures and preliminarily tested by C.A.S. reagent and UV. The siderophores activity against E. coli O157:H7 was characterised by the determination of Minimal Inhibiting Concentration (MIC). Furthermore, E. coli O157:H7 growth tests with and without siderophores were prepared at 12°C, pH 7 and 5, aw 0.997 and 0,988. The MIC was assessed at 7 μg/ml whereas the growth test showed a high inhibiting activity with the exception of tests at pH 5