
COMPARISON BETWEEN TETRACYCLINE RESISTANT ENTEROCOCCI ISOLATED FROM SHEEP AND TYPICAL CHEESE IN THE GEOGRAPHICAL AREA OF “PARCO NAZIONALE DEI MONTI SIBILLINI”
Author(s) -
Valentina Cambiotti,
D. Garofalo,
Beniamino T. CenciGoga
Publication year - 2012
Publication title -
italian journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 13
ISSN - 2239-7132
DOI - 10.4081/ijfs.2012.4.43
Subject(s) - tetracycline , biology , microbiology and biotechnology , antibiotic resistance , bacteria , feces , antibiotics , microorganism , veterinary medicine , food science , genetics , medicine
Enterococci were isolated from sheep faeces and cheese taken in the «Parco nazionale dei monti Sibillini»; then they were submitted to the antibiotic susceptibility test and to the PCR reaction to amplify tet M and tet O genes. The analysis showed that 23,64% of Enterococci from sheep and 30,77% from cheese were resistant to tetracycline; 14 strains from sheep and 5 from cheese contained tet M and tet O genes as genetic determinants of resistance. These results allow to say that resistant bacteria and genes have the possibility to be transferred from animals to derived food; therefore reducing antibiotic use in farm animals could affect the amount of resistant microorganisms that can reach human beings using this way of diffusion