Open AccessIDENTIFICATION OF E. COLI O157 IN A BOVINE MILK FARM BY MULTIPLEX REAL-TIME PCR
Author(s) -
Annalisa Petruzzelli,
Giulia Amagliani,
Martina Foglini,
Chiara Bartolini,
F. Agnetti,
Chiara Francesca Magistrali,
Enrica Omiccioli,
Giorgio Brandi,
Francesco Tonucci
Publication year - 2012
Publication title -
italian journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 13
ISSN - 2239-7132
DOI - 10.4081/ijfs.2012.4.105
Subject(s) - vtec , raw milk , listeria monocytogenes , bovine milk , multiplex polymerase chain reaction , salmonella , biology , food science , foodborne pathogen , identification (biology) , real time polymerase chain reaction , sheep milk , feces , multiplex , microbiology and biotechnology , veterinary medicine , polymerase chain reaction , escherichia coli , bacteria , medicine , bioinformatics , genetics , botany , gene
Law provisions about direct sell of raw bovine milk require VTEC O157 monitoring in bovine milk farms (milk and faeces). It has been showed that culture-based methods used for this scope, besides being cumbersome and time-consuming, may be also less sensitive, compared to molecular approaches. In this study, a multiplex Real-Time PCR, able to identify VTEC O157, Salmonella spp and Listeria monocytogenes, has been used to analyse milk, filter, sewage and stool samples from a milk farm, in comparison with standard OIE methods. The performances of the molecular protocol have been preliminary assessed with lyophilized samples from proficiency testing VLA, showing 100% accordance. Results from field samples indicated the absence of the pathogen in milk, and the higher sensitivity of Real-Time PCR with other matrices, suggesting its potential use for fast VTEC O157 identification