Open AccessMOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY
Author(s) -
Annalisa Petruzzelli,
Floriana Ciarrocchi,
Martina Foglini,
Stefano Fisichella,
Carla Sebastiani,
Wally Baffone,
Sara Santarelli,
Francesco Tonucci,
Giuliana Blasi
Publication year - 2011
Publication title -
italian journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.359
H-Index - 13
ISSN - 2239-7132
DOI - 10.4081/ijfs.2011.1s.287
Subject(s) - upgma , rapd , genotyping , biology , genotype , population , genetic diversity , genetics , microbiology and biotechnology , gene , medicine , environmental health
The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and culturebased methods. The estimation of genotypic intra-species variation of the autochthonous bacteria isolated was also evaluated by using randomly amplified polymorphic DNA (RAPD) analysis and unweighted pairgroup method with arithmetic averages (UPGMA) cluster analysis. Our findings revealed an high diversity of the autochthonous bacterial population investigated, both at species and strain level