Open AccessOptimization of primary hepatocyte isolation for the pharmacological characterization of metabotropic glutamate receptor (mGluR) s ubtype 5: A study on Reduction
Author(s) -
Clarissa Berardo,
Andrea Ferrigno,
Veronica Siciliano,
Marta Cagna,
Plinio Richelmi,
Mariapia Vairetti,
Laura Giuseppina Di Pasqua
Publication year - 2021
Publication title -
biomedical science and engineering
Language(s) - English
Resource type - Journals
ISSN - 2531-9892
DOI - 10.4081/bse.2021.151
Subject(s) - collagenase , metabotropic glutamate receptor 5 , metabotropic glutamate receptor 1 , metabotropic glutamate receptor , metabotropic glutamate receptor 2 , metabotropic glutamate receptor 7 , metabotropic glutamate receptor 6 , metabotropic receptor , metabotropic glutamate receptor 4 , metabotropic glutamate receptor 3 , chemistry , receptor , hepatocyte , glutamate receptor , reduction (mathematics) , pharmacology , biochemistry , microbiology and biotechnology , biology , enzyme , mathematics , geometry , in vitro
To minimize the number of animals used during experiments, it is important to choose the suitable enzyme according to the final goal. In our work, we demonstrated the superiority of collagenase IV in the maintenance of functional transmembrane receptor, thus the pharmacological activity, in isolated rat hepatocytes.