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open-access-imgOpen AccessDNA aberrations in urinary bladder cancer detected by flow cytometry and fish: prognostic implications
Author(s)
A. M. Cianciulli,
R. Bovani,
C. Leonardo,
F. Iori,
Coletta Am,
Raffaella Marzano,
Anna Antenucci,
Giuseppe Maria Gandolfo,
C. Laurenti
Publication year2009
Publication title
european journal of histochemistry
Resource typeJournals
PublisherPAGEPress (Italy)
We evaluated the genetic changes in bladder cancer biopsy by fluorescence in situ hybridization (FISH) and related them to stage and grade of the tumor, ploidy (FCM) and clinical outcome, to determine a simple method to identify tumors with a poorer prognosis. Using FISH the numerical aberrations of chromosomes 1,7,9,17 in tumor’s imprints of 70 patients with transitional cell cancer (TCC) were determined. First of all, the data demonstrated that the sensitivity of FISH in detecting quantitative DNA aberrations exceeds FCM’s sensitivity. The frequency of chromosome 1 and 9 aberrations did not show significant differences in diploid and aneuploid tumors in different stage and grade. On the contrary, the chromosome 7 and 17 aneusomy showed greater differences between pT1 and pT2-3 tumors (p<0.032 and p<0.0006, respectively) than between stage pTa and pT1. In our investigation, an increasing number of aberrations was observed in all chromosomes examined in tumors of patients who afterwards underwent cystectomy and/or had recurrent tumors. These results suggest that chromosome 7 and 17 aneusomy could be predictive of adverse outcome in a subgroup of patients with superficial tumors at presentation
Subject(s)aneuploidy , biology , biopsy , bladder cancer , cancer , chromosome , cystectomy , fish <actinopterygii> , fishery , flow cytometry , fluorescence in situ hybridization , gene , genetics , medicine , microbiology and biotechnology , oncology , paleontology , pathology , ploidy , stage (stratigraphy) , urinary bladder
Language(s)English
SCImago Journal Rank0.754
H-Index42
eISSN2038-8306
pISSN1121-760X
DOI10.4081/1615

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