Open AccessSimultaneous presence of Paralytic and Diarrheic Shellfish Poisoning toxins in Mytilus chilensis samples collected in the Chiloe Island, Austral Chilean Fjords
Author(s) -
Carlos Garcı́a,
Pamela Mardones,
Andrea Sfeir,
Néstor Lagos
Publication year - 2004
Publication title -
biological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.127
H-Index - 55
eISSN - 0717-6287
pISSN - 0716-9760
DOI - 10.4067/s0716-97602004000500002
Subject(s) - mussel , paralytic shellfish poisoning , shellfish , shellfish poisoning , mytilus , dinophysis , fjord , marine toxin , water column , biology , algal bloom , environmental chemistry , toxin , fishery , chromatography , zoology , phytoplankton , chemistry , microbiology and biotechnology , aquatic animal , ecology , oceanography , nutrient , fish <actinopterygii> , geology
The study shown here provides the first indisputable evidence that shellfish can be contaminated with Paralytic Shellfish Poisoning (PSP) and Diarrheic Shellfish Poisoning (DSP) toxins during the summer season in the Southern Chilean fjords. Quantitative analysis of the simultaneous presence of PSP and DSP toxins in Mytilus chilensis samples collected in the Chiloe Island are shown. The High Performance Liquid Chromatography (HPLC) analysis with pre-column derivatization method for DSP toxins and the post-column derivatization methods for PSP toxins, both with fluorescent on-line detections, showed that both type of toxins were concentrated by the filter bivalve Mytilus chilensis in amounts above the international safe limits. The phytoplankton analysis showed the presence of both Alexandrium catenella and Dinophysis acuta in the water column. The data shows stratification of the toxic dinoflagellates in the water column, since the lowest amount of both DSP and PSP toxins were measured in the superficial and deeper levels of the water column. Moreover, the highest toxicities of both types of toxins were shown by the shellfish samples collected at a depth of 6 meters with 190 nanograms of DTX-1 / gram of digestive gland and 709.8 microg of PSP toxins / 100 grams of mussel meat.