Open AccessAnti-inflammatory activity of two varieties of pumpkin seed oil in an adjuvant arthritis model in rats
Author(s) -
Sahar Y. AlOkbi,
Doha A. Mohamed,
Eman Kandil,
Mona A.M. Abo-Zeid,
Shaimaa Mohammed,
Emad K. Ahmed
Publication year - 2017
Publication title -
grasas y aceites
Language(s) - English
Resource type - Journals
eISSN - 1988-4214
pISSN - 0017-3495
DOI - 10.3989/gya.0796161
Subject(s) - malondialdehyde , oxidative stress , arthritis , antioxidant , chemistry , necrosis , andrology , immunology , pathology , medicine , biochemistry
The aim of the present research was to evaluate the anti-inflammatory activity of pumpkin seed oils (PSOs) of an Egyptian and European variety, in a rat model of adjuvant arthritis. Edema thickness, plasma tumor necrosis factor-α (TNF-α) and erythrocyte sedimentation rate (ESR) were determined as inflammatory biomarkers while malondialdehyde (MDA) and total antioxidant capacity (TAC) were assessed as indicative of oxidative stress. Chromosomal aberration, sperm shape abnormalities, and DNA fragmentations are cytogenetic parameters which aid in tracing inflammatory and oxidative activity. Phenolic contents and β-carotene were determined in PSOs. The results showed elevated ESR, plasma TNF-α, plasma MDA, liver cellular DNA fragmentation, bone marrow chromosomal aberration, sperm shape abnormalities with a reduction in plasma TAC and body weight gain in an adjuvant arthritis control compared to a healthy control. Administration of low and high doses of either Egyptian or European PSO improved all the aforementioned parameters with variable degrees