Open AccessCartilage type IIB procollagen NH<sub>2</sub>-propeptide, PIIBNP, inhibits angiogenesis
Author(s) -
Zhepeng Wang,
AUTHOR_ID,
Aiwu Lu,
AUTHOR_ID
Publication year - 2021
Publication title -
aims molecular science
Language(s) - English
Resource type - Journals
eISSN - 2372-028X
pISSN - 2372-0301
DOI - 10.3934/molsci.2021022
Subject(s) - angiogenesis , procollagen peptidase , microbiology and biotechnology , chemistry , integrin , umbilical vein , cartilage , biology , in vitro , cell , cancer research , biochemistry , anatomy
Cartilage tissue is avascular and resistant to tumor invasion, but the basis for these properties is still unclear. Here we report that the NH 2 -propeptide of type IIB procollagen (PIIBNP), a product of collagen biosynthesis, is capable of inhibiting angiogenesis both in vitro and in vivo . PIIBNP inhibits tube formation in human umbilical vein cells (HUVEC), inhibits endogenous endothelial cell outgrowth in mouse aortic ring angiogenesis bioassay and is anti-angiogenic in the mouse cornea angiogenesis assay. As α V ß 3 and α V ß 5 integrins are expressed primarily in endothelial cells, cancer cells and osteoclasts, but not in normal chondrocytes and PIIBNP binds to cell surface integrin α V ß 3 and αVß 5 , we propose that natural occurring PIIBNP protects cartilage by targeting endothelial cells during chondrogenesis, thus inhibiting angiogenesis, and rendering the tissue avascular.