Open AccessHPLC-MS identification and expression of <i>Candida</i> drug-resistance proteins from African HIV-infected patients
Author(s) -
Pedro Miguel dos Santos Abrantes,
Randall G. Fisher,
Patrick Bouic,
Carole McArthur,
Burtram C. Fielding,
Charlene Wilma Joyce Africa
Publication year - 2021
Publication title -
aims microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.565
H-Index - 6
ISSN - 2471-1888
DOI - 10.3934/microbiol.2021020
Subject(s) - efflux , biology , drug resistance , candida albicans , proteome , drug , abcg2 , microbiology and biotechnology , multiple drug resistance , downregulation and upregulation , transporter , heat shock protein , corpus albicans , gene , atp binding cassette transporter , pharmacology , biochemistry
The objective of this study was to elucidate the proteomic mechanisms of drug resistance in HIV-infected African patients. Cell membrane fractions from forty oral Candida isolates isolated from African HIV-positive patients were analysed using HPLC-MS with the aim of identifying proteins associated with their pathogenicity and drug resistance. Heat shock proteins that mediate the fungicidal activity of salivary peptides were found in all tested Candida fractions, with pH-responsive proteins associated with increased pathogenicity only being present in the three most commonly isolated species. ABC multidrug transporter efflux pumps and estrogen binding proteins were only found in C. albicans fractions, while ergosterol biosynthesis proteins were identified in four species. The combination of various adherence, invasion, upregulation and efflux pump mechanisms appear to be instrumental for the Candida host colonization and drug resistance emergence in HIV-infected individuals.