Open AccessIntrinsic role of coagulase negative staphylococci <em>norA</em>-like efflux system in fluoroquinolones resistance
Author(s) -
Marco Ligozzi,
Liliana Galia,
Anna Bertoncelli,
Annarita Mazzariol
Publication year - 2017
Publication title -
aims microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.565
H-Index - 6
ISSN - 2471-1888
DOI - 10.3934/microbiol.2017.4.908
Subject(s) - efflux , coagulase , microbiology and biotechnology , chemistry , staphylococcus , biology , staphylococcus aureus , biochemistry , genetics , bacteria
NorA is a Staphylococcus aureus multidrug transporter that exports structurally distinct compounds including fluoroquinolones. In this study norA- like genes of Staphylococcus epidermidis ( norA SEP ) and Staphylococcus haemolyticus ( norA SHAE ) were identified and sequenced. The nucleotide identity of norA SEP and norA SHAE with norA was 75.3 and 74.1%, respectively, and the amino acid identity 87.7 and 86%, respectively. Inactivation of norA SEP increased the ciprofloxacin susceptibility of E. coli DH5α carrying the pB SK 198 norA SEP EZ cat norA SEP plasmid.