Open AccessOil-in-water emulsion formulated with eucalyptus leaves extract inhibit influenza virus binding and replication in vitro
Author(s) -
Maryam Sadat Sadatrasul,
Neda Fiezi,
Nasir Ghasemian,
Mohammad Shenagari,
Saber Esmaeili,
Ehsan Ollah Jazaeri,
Asghar Abdoli,
Abbas Jamali
Publication year - 2017
Publication title -
aims microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.565
H-Index - 6
ISSN - 2471-1888
DOI - 10.3934/microbiol.2017.4.899
Subject(s) - eucalyptus oil , virus , titer , virology , microbiology and biotechnology , in vitro , infectious dose , hemagglutination assay , biology , viral replication , chemistry , immunology , biochemistry
Throughout human history, the human-beings have been used different types of plants as antimicrobial agents in fight against infectious diseases. Influenza virus is one of the most common causes of respiratory infection and transmitted through direct contact with flu infected individuals and contaminated substances or droplets. In the current study, both oil-in-water and water-in-oil emulsions with hydroalcoholic extract of eucalyptus leaves (OLHE) were developed and their antiviral efficiency was evaluated. To doing so, Madin-Darbey Canine Kidney (MDCK) cells were treated with effective minimal cytotoxic concentration of the formulated emulsions. The treated cells were then infected with 50% cell culture infectious dose (100 CCID 50 ) of the A/H1N1 virus (the swine flu). The viral titers were measured by hemagglutination (HA) and cell culture infectious dose 50% (CCID 50 ) assays. Also, to check the virus binding inhibition via the formulated extract, the viruses were incubated with the formulated extracts. Our study showed that the oil-in-water emulsions formulated with 2% eucalyptus leaves extract inhibited virus replication completely when the cells were infected by 100 CCID 50 and decreased HA titer up to four fold. Therefore, this formulation, may hold promising application to prevent influenza virus transmission through direct contact among children and passengers.