RHAMM regulates the growth and migration of lung adenocarcinoma A549 cell line by regulating Cdc2/CyclinB1 and MMP9 genes | Zendy

Feng Chen | Zendy; Xuqing Zhu | Zendy; Jing Zheng | Zendy; Ting-Ting Xu | Zendy; Kuan Wu | Zendy; Chuhui Ru | Zendy

Open Access

RHAMM regulates the growth and migration of lung adenocarcinoma A549 cell line by regulating Cdc2/CyclinB1 and MMP9 genes

Author(s) -

Feng Chen,

Xuqing Zhu,

Jing Zheng,

Ting-Ting Xu,

Kuan Wu,

Chuhui Ru

Publication year - 2020

Publication title -

mathematical biosciences and engineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.451

H-Index - 45

eISSN - 1551-0018

pISSN - 1547-1063

DOI - 10.3934/mbe.2020114

Subject(s) - a549 cell , cell cycle , cell growth , cyclin dependent kinase 1 , cell culture , gene silencing , cell migration , microbiology and biotechnology , cancer research , cd44 , gene knockdown , epithelial–mesenchymal transition , chemistry , flow cytometry , biology , cell , downregulation and upregulation , genetics , biochemistry , gene

Objective : The study aims to explore the effects of receptor of hyaluronan mediated motility ( RHAMM ) on the proliferation, invasion and migration of the lung adenocarcinoma (LUAD) cell line A549 and its targeted regulatory pathway. Methods : Bioinformatics was used to analyze the differentially expressed genes in LUAD chips. The mRNA and protein expression level of Cdc2, CyclinB1, MMPs and epithelial-mesenchymal transition (EMT) related markers E-cadherin and Vimentin were tested by qRT-PCR and western blot in A549 cell line after silencing RHAMM . Cell proliferation, cell division cycle, migration and invasion abilities were tested in RHAMM knockdown A549 cells by flow cytometry and in vitro assays. Results : Silencing RHAMM inhibited EMT, proliferation, migration and invasion of A549 cell line and induced cells to cluster at G2/M phase. In addition, after silencing RHAMM , the mRNA and protein expressions of Cdc2 and CyclinB1 were decreased while those of MMP9 were increased. Conclusion : The findings suggest tha RHAMM regulates cell division cycle by regulating Cdc2 and CyclinB1 , and regulates extracellular matrix degradation by regulating MMP9 . These targeted modulations regulate the occurrence and development of LUAD cells.

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