Open AccessMolecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae
Author(s) -
Tahereh Karamzadeh,
Hamzeh Alipour,
Marziae Shahriari-Namadi,
Abbasali Raz,
Kourosh Azizi,
Masoumeh Bagheri,
Mohammad Djaefar MoemenbellahFard
Publication year - 2019
Publication title -
aims genetics
Language(s) - English
Resource type - Journals
ISSN - 2377-1143
DOI - 10.3934/genet.2019.3.46
Subject(s) - lucilia , netrin , larva , biology , zoology , anatomy , genetics , receptor , calliphoridae , ecology , axon guidance
Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1 , one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor . The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5 . It lied at the interval of 26-131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata . It could be applied to research activities implementing a new essential component design in wound healing.