Open AccessRETRACTED: Thermal investigation of montmorillonite/BSA by Fourier Transform InfraRed Spectroscopy measurements
Author(s) -
Maria Teresa Caccamo,
AUTHOR_ID,
Giuseppe Mavilia,
Letterio Mavilia,
Pietro Calandra,
Domenico Lombardo,
Salvatore Magazù,
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AUTHOR_ID,
AUTHOR_ID
Publication year - 2020
Publication title -
aims biophysics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.545
H-Index - 12
ISSN - 2377-9098
DOI - 10.3934/biophy.2020019
Subject(s) - montmorillonite , fourier transform infrared spectroscopy , thermal stability , infrared spectroscopy , bovine serum albumin , infrared , spectroscopy , materials science , analytical chemistry (journal) , intramolecular force , thermal analysis , chemistry , thermal , chemical engineering , physics , stereochemistry , organic chemistry , thermodynamics , chromatography , optics , composite material , quantum mechanics , engineering
This paper reports the analysis of the intramolecular OH stretching band obtained by Fourier Transform Infrared (FTIR) spectroscopy measurements. In order to characterize the effect of montmorillonite on the properties of Bovine Serum Albumin (BSA) the two-state model is adopted for the analysis of the OH stretching band. We assume that the OH stretching can be divided into two different states of inter-molecular bonding. The results of this experimental work confirm that the montmorillonite leads to a stabilization of the BSA structure. Also, the analysis of the spectra temperature dependence shows a montmorillonite-induced higher thermal stability of the BSA in respect to pristine BSA. Thus, this paper allows to highlight the importance of montmorillonite as thermal bio-protector: this is also evidenced by the theory widely discussed in the following introduction regarding the birth of the first life forms on earth in montmorillonite clay, in which the protective role of the montmorillonite interlayer space is also highlighted. A FTIR analysis was carried out to investigate the interaction of montmorillonite with BSA. Two different approaches, i.e. Spectral Distance and Wavelet analyses, constitute two effective and innovative approaches for the characterization of the thermal properties of pristine BSA and of BSA in the presence of montmorillonite. The results allowed us to consider as BSA in the presence of montmorillonite has a lower spectral sensitivity when the temperature changes and, therefore, the role of montmorillonite as a thermal bio-protector is motivated.