Open AccessSingle cell adhesion strength assessed with variable-angle total internal reflection fluorescence microscopy
Author(s) -
Marcelina Cardoso Dos Santos,
Cyrille Vézy,
Hamid Morjani,
Rodolphe Jaffol
Publication year - 2017
Publication title -
aims biophysics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.545
H-Index - 12
ISSN - 2377-9098
DOI - 10.3934/biophy.2017.3.438
Subject(s) - total internal reflection fluorescence microscope , membrane , total internal reflection , adhesion , microscopy , biophysics , materials science , dissociation (chemistry) , fluorescence microscope , cell adhesion , substrate (aquarium) , analytical chemistry (journal) , fibronectin , fluorescence , chemistry , cell , composite material , optics , chromatography , biology , optoelectronics , biochemistry , physics , ecology
We propose a new strategy to evaluate adhesion strength at the single cell level. This approach involves variable-angle total internal reflection fluorescence microscopy to monitor in real time the topography of cell membranes, i.e. a map of the membrane/substrate separation distance. According to the Boltzmann distribution, both potential energy profile and dissociation energy related to the interactions between the cell membrane and the substrate were determined from the membrane topography. We have highlighted on glass substrates coated with poly-L-lysine and fibronectin, that the dissociation energy is a reliable parameter to quantify the adhesion strength of MDA-MB-231 motile cells