Open AccessMinimizing <em>Pseudomonas aeruginosa</em> adhesion to titanium surfaces by a plasma nitriding process
Author(s) -
Michelle de Medeiros Aires,
Janine Treter,
Antônio Nunes Filho,
Igor Oliveira Nascimento,
Alexandre José Macêdo,
Clodomiro Alves Júnior
Publication year - 2016
Publication title -
aims biophysics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.545
H-Index - 12
ISSN - 2377-9098
DOI - 10.3934/biophy.2017.1.19
Subject(s) - nitriding , titanium , biofilm , pseudomonas aeruginosa , materials science , titanium nitride , adhesion , surface modification , surface roughness , scanning electron microscope , bacteria , nuclear chemistry , chemical engineering , chemistry , metallurgy , layer (electronics) , nitride , nanotechnology , composite material , biology , engineering , genetics
The research of the interaction between bacteria-surface has great importance for titanium biomedical applications once microorganisms offer risks because promoting implant loss. Therefore, study bacterial adhesion and colonization on titanium is interesting because are principal factors infections pathogeny on biomaterials. In this study, commercial grade II titanium was submitted to nitriding treatment to plasma at 2.2 mbar, using gas mixtures of 80% hydrogen (H2) and 20% nitrogen (N2) during 1 hour and 3 hour. The surfaces were physically and chemically characterized. In order to evaluate bacterial response, the surfaces were exposed to Pseudomonas aeruginosa. The titanium surface modified in nitriding plasma, although exposes a higher roughness as compared with untreated samples, exhibited lower bacterial growth. The nitrided sample for 3 hour exhibited the higher amount of TiN phase and the higher concentration of atomic nitrogen on surface and lower bacterial adhered count. These results were confirmed by scanning electron microscopy. Based on these results can be said to the thermochemical treatment of plasma nitriding on titanium samples results a significant reduction of adherence of Pseudomonas aeruginosa. It was found that the Ti surface nitrided offers significant reduction of bacterial adherence which prevent biofilm formation and offersing lower risk of infection and implant remotion