Open AccessSpatial mechanistic modeling for prediction of 3D multicellular spheroids behavior upon exposure to high intensity pulsed electric fields
Author(s) -
Annabelle Collin,
Hadrien Bruhier,
Jelena Kolosnjaj,
Muriel Golzio,
Marie-Pierre Rols,
Clair Poignard
Publication year - 2022
Publication title -
aims bioengineering
Language(s) - English
Resource type - Journals
ISSN - 2375-1495
DOI - 10.3934/bioeng.2022009
Subject(s) - spheroid , electroporation , multicellular organism , electric field , biophysics , irreversible electroporation , fluorescence microscope , chemistry , biological system , biology , cell , fluorescence , optics , physics , in vitro , biochemistry , quantum mechanics , gene
The objective of this work was to investigate the growth specificities of cancer cells spheroids subjected to pulsed electric field. Multicellular HCT-116-GFP spheroids were exposed to different electric field intensities and the volume of multicellular spheroids was monitored by fluorescence and bright field microscopy. Thanks to an advanced mathematical model, based on differential equations and well-adapted estimation strategies, our modeling enables us to characterize the multicellular spheroids growth after permeabilizing pulsed electric field. In particular, we identify the percentage of cells which are destroyed and the percentage of cells which exhibit an altered growth pattern for different magnitudes of the electric field. We also quantify the growth resumption upon reversible and partially irreversible electroporation. Our preliminary results provide a first quantification of the impact of electroporation on multicellular spheroids growth, and suggest a booming growth of partially irreversible electric pulses, leading to an accelerated regrowth.