Simple Colorimetric Method for Cholinesterase-inhibitor Screening in Gastric Content by Using Phytoesterase Enzyme from Kidney Bean | Zendy

Thanawat Kaewmaroen | Zendy; Klintean Wunnapuk | Zendy; Manoch Chockjamsa | Zendy; Korawan Sringarm | Zendy; Surat Hongsibson | Zendy

Open Access

Simple Colorimetric Method for Cholinesterase-inhibitor Screening in Gastric Content by Using Phytoesterase Enzyme from Kidney Bean

Author(s) -

Thanawat Kaewmaroen,

Klintean Wunnapuk,

Manoch Chockjamsa,

Korawan Sringarm,

Surat Hongsibson

Publication year - 2020

Publication title -

pakistan journal of biological sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.268

H-Index - 43

eISSN - 1812-5735

pISSN - 1028-8880

DOI - 10.3923/pjbs.2020.904.910

Subject(s) - cholinesterase , chemistry , simple (philosophy) , kidney , enzyme , chromatography , biochemistry , food science , pharmacology , medicine , endocrinology , philosophy , epistemology

Diagnosis of cholinesterase inhibitor insecticide ingestion is based on clinical suspicious and should be confirmed by cholinesterase essay. However, serum cholinesterase activity test requires specific instruments and procedure. This study aimed to develop simple colorimetric test to detect cholinesterase inhibitors in the gastric content, using phytoesterase and alpha naphthyl acetate as a chromogenic substrate.

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