Open AccessImmunomagnetic separation and Listeriamonocytogenes detection with surface-enhanced Raman scattering
Author(s) -
Hande Yeğenoğlu Akçinar,
Belma Aslım,
Hilal Torul,
Burcu Güven,
Adem Zengi̇n,
Zekiye Suludere,
İsmail Hakkı Boyacı,
Uğur Tamer
Publication year - 2020
Publication title -
turkish journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.277
H-Index - 27
eISSN - 1303-6165
pISSN - 1300-0144
DOI - 10.3906/sag-2002-234
Subject(s) - listeria monocytogenes , detection limit , immunomagnetic separation , chromatography , staphylococcus aureus , population , raman spectroscopy , enumeration , biocompatibility , nanoparticle , microbiology and biotechnology , bacteria , medicine , chemistry , materials science , nanotechnology , biology , mathematics , genetics , physics , environmental health , organic chemistry , combinatorics , optics
Background/aim We aimed to develop a rapid method to enumerate Listeria monocytogenes ( L. monocytogenes ) utilizing magnetic nanoparticle based preconcentration and surface-enhanced Raman spectroscopy measurements. Materials and methods Biological activities of magnetic Au-nanoparticles have been observed to have the high biocompatibility, and a sample immunosensor model has been designed to use avidin attached Au-nanoparticles for L. monocytogenes detection. Staphylococcus aureus ( S. aureus ) and Salmonella typhimurium ( S. typhimurium ) bacteria cultures were chosen for control studies. Antimicrobial activity studies have been done to identify bio-compatibility and bio-characterization of the Au-nanoparticles in our previous study and capturing efficiencies to bacterial surfaces have been also investigated.Results We constructed the calibration graphs in various population density of L. monocytogenes as 2.2 × 101 to 2.2 × 106 cfu/mL and the capture efficiency was found to be 75%. After the optimization procedures, population density of L. monocytogenes and Raman signal intensity showed a good linear correlation (R2 = 0.991) between 102 to 106 cfu/mL L. monocytogenes . The presented sandwich assay provides low detection limits and limit of quantification as 12 cfu/mL and 37 cfu/mL, respectively. We also compared the experimental results with reference plate-counting methods and the practical utility of the proposed assay is demonstrated using milk samples. Conclusion It is focused on the enumeration of L. monocytogenes in milk samples and the comparision of results of milk analysis obtained by the proposed SERS method and by plate counting method stay in food agreement. In the present study, all parameters were optimized to select SERS-based immunoassay method for L. monocytogenes bacteria to ensure LOD, selectivity, precision and repeatablity.