Open AccessOptimization of isothermal amplification method for Mycobacteriumtuberculosisdetection and visualization method for fieldwork
Author(s) -
Hatice Esra Ağel,
Hasan Sağcan,
İsmail Ceyhan,
Rıza Durmaz
Publication year - 2020
Publication title -
turkish journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.277
H-Index - 27
eISSN - 1303-6165
pISSN - 1300-0144
DOI - 10.3906/sag-1910-6
Subject(s) - loop mediated isothermal amplification , agarose gel electrophoresis , primer (cosmetics) , polymerase chain reaction , microbiology and biotechnology , agarose , recombinase polymerase amplification , mycobacterium tuberculosis , detection limit , taqman , chromatography , chemistry , dna , biology , tuberculosis , medicine , gene , biochemistry , pathology , organic chemistry
Tuberculosis is still one of the most contagious diseases around the world. Key factors of tuberculosis control are rapid diagnostic, efficient treatment, and prevention of contamination by surveillance and monitoring. However, culture is the gold standard method for laboratory diagnosis of tuberculosis; the results are several weeks to obtain. In order to prevent contamination of tuberculosis, diagnosis must be made in short time and treatment should be started as soon as possible. The aim of this study is to optimize the loop-mediated isothermal amplification (LAMP) method, which provides a much faster and more sensitive result than the polymerase chain reaction (PCR) method and allows the replication of target nucleic acid sequences under isothermal conditions without the need for laboratory infrastructure.