Open AccessTransplantation of undifferentiated bone marrow stromal cells improves sciatic nerve regeneration and functional recovery through inside-out vein graft in rats
Author(s) -
Rahim Mohammadi,
Saeed Azizi,
Nowruz Delirezh,
Rahim Hobbenaghi,
Keyvan Amini
Publication year - 2012
Publication title -
turkish journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.277
H-Index - 27
eISSN - 1303-6165
pISSN - 1300-0144
DOI - 10.3906/sag-1009-1174
Subject(s) - medicine , sciatic nerve , regeneration (biology) , stromal cell , schwann cell , transplantation , bone marrow , nerve guidance conduit , pathology , surgery , andrology , anatomy , microbiology and biotechnology , biology
doi:10.3906/sag-1009-1174 Transplantation of undiff erentiated bone marrow stromal cells improves sciatic nerve regeneration and functional recovery through inside-out vein graft in rats Rahim MOHAMMADI1, Saeed AZIZI1, Nowruz DELIREZH2, Rahim HOBBENAGHI3, Keyvan AMINI4 Aim: To study the eff ect of undiff erentiated bone marrow stromal cells (BMSCs) on peripheral nerve regeneration using a rat sciatic nerve regeneration model. In recent years, cell transplantation has become the focus of attention, and reliable outcomes have been achieved in regeneration of the sciatic nerve. Materials and methods: A 10-mm sciatic nerve defect was bridged using an inside-out vein graft (IOVG) fi lled with undiff erentiated BMSCs (2 × 107 cells/mL). In the control group, the vein was fi lled with phosphate buff ered saline alone. Th e regenerated fi bers were studied 4, 8, and 12 weeks aft er surgery. Assessment of nerve regeneration was based on functional (walking track analysis), histomorphometric, and immunohistochemical (Schwann cell detection by S-100 expression) criteria. Results: Th e functional study confi rmed signifi cant recovery of regenerated axons in the IOVG/BMSC group (P < 0.05). Quantitative morphometric analyses of regenerated fi bers showed that the number and diameter of myelinated fi bers i