Open AccessIn vitro effect of resveratrol against oxidative injury of human coronary artery endothelial cells
Author(s) -
Oya Sayın,
Nur Arslan,
Zekiye Altun,
Gül Güner Akdoğan
Publication year - 2011
Publication title -
turkish journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.277
H-Index - 27
eISSN - 1303-6165
pISSN - 1300-0144
DOI - 10.3906/sag-0907-128
Subject(s) - resveratrol , reactive oxygen species , oxidative stress , hydrogen peroxide , medicine , antioxidant , endothelial dysfunction , endothelial stem cell , lactate dehydrogenase , pharmacology , endothelium , biochemistry , in vitro , chemistry , enzyme
doi:10.3906/sag-0907-128 In vitro eff ect of resveratrol against oxidative injury of human coronary artery endothelial cells Oya SAYIN1,2,3, Nur ARSLAN2,3, Zekiye Sultan ALTUN4, Gül AKDOĞAN1,2,3 Aim: Reactive oxygen species induce oxidative stress that may contribute to endothelial dysfunction. In addition to endogen antioxidants, adding antioxidants to diet has been used to reduce oxidative-stress-induced endothelial dysfunction. Resveratrol is a natural phytoalexin which has many eff ects including antioxidant activity. Th e aim of this study was to investigate whether resveratrol could protect human coronary artery endothelial cells against in vitro oxidative-stress-mediated injury. Materials and methods: Human coronary artery endothelial cells were treated with varying concentrations of hydrogen peroxide for diff erent durations and hydrogen peroxide plus varying concentrations of resveratrol. Th en cell death was measured using lactate dehydrogenase release. Human coronary artery endothelial cells were exposed to hydrogen peroxide and resveratrol plus hydrogen peroxide for 15 min, 60 min, and 6 h. Reactive oxygen species was measured using a fl uorometric assay. Results: Preincubation of cells with 10 and 50 μM resveratrol for diff erent periods caused a signifi cantly decreased hydrogen-peroxide-induced cell death. Preincubation with resveratrol caused a signifi cant decrease in reactive oxygen species production. Conclusion: Our results demonstrate that resveratrol protects human coronary artery endothelial cells, in vitro, against oxidative-stress-induced injury by decreasing cell death and reactive oxygen species production. Key words: Resveratrol, human coronary artery endothelial cell, oxidative stress Resveratrolün insan koroner arter endotel hücrelerinde oksidatif hücre hasarina karşı in vitro etkisi Amaç: Reaktif oksijen ürünleri endotel fonksiyon kaybına katkıda bulunabilen oksidatif stresi indüklemektedir. Endojen antioksidanların yanı sıra, diyetle antioksidanların alımı, endotel fonksiyon kaybını indükleyen oksidatif stresi azaltmaktadır. Resveratrol antioksidan aktiviteyi kapsayan birçok etkilere sahip doğal bir fi toaleksindir. Bu çalışmanı