Open AccessCytotoxic effects of caffeic acid phenethyl ester (CAPE) on the human multiple myeloma cell line
Author(s) -
Özgür Koru,
Ferit Avcu,
Mehmet Tanyüksel,
Ali Uğur Ural,
Remzi Engin Araz,
Kenan Şener
Publication year - 2009
Publication title -
turkish journal of medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.277
H-Index - 27
eISSN - 1303-6165
pISSN - 1300-0144
DOI - 10.3906/sag-0805-42
Subject(s) - caffeic acid phenethyl ester , cytotoxic t cell , apoptosis , cytotoxicity , trypan blue , cell culture , chemistry , propolis , cape , microbiology and biotechnology , multiple myeloma , in vitro , pharmacology , biochemistry , biology , immunology , caffeic acid , antioxidant , food science , history , archaeology , genetics
doi:10.3906/sag-0805-42 Cytotoxic effects of caffeic acid phenethyl ester (CAPE) on the human multiple myeloma cell line*,** Aim: Caffeic acid phenethyl ester (CAPE) has cytotoxic, apoptotic, and antiproliferative effects on various tumor cells, and is the most active component of propolis. This study aimed to examine the in vitro effects of CAPE on the human multiple myeloma cell line. Materials and methods: CAPE was added to the ARH-77 multiple myeloma cell line and the percentage of dead cells was measured using the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-SH-tetrazolium bromide (MTT) assay. The percentage of live cells and growth inhibition were determined using the Trypan blue test. The percentage of IL-6 cells was determined using ELISA. Results: ARH-77 cells treated with CAPE for 72 h at the 100 μg mL−1 concentration resulted in a growth inhibition effect of 90.4 % and a cytotoxic effect of 80.4%. CAPE induced apoptosis in 92.3% of the cells in 22.5 μg mL−1 at 72 h. CAPE inhibited the secretion of IL-6 by ARH-77 multiple myeloma cells at LD50 concentrations. Conclusion: CAPE inhibited growth and secretion of IL-6, and induced apoptosis in a dose