Open AccessAnti-infective properties of ursolic acid-loaded chitosan nanoparticles against Staphylococcus aureus
Author(s) -
Fatemeh Ghasemzadeh,
Ghasem Najafpour,
Maedeh Mohammadi
Publication year - 2021
Publication title -
turkish journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.239
H-Index - 46
eISSN - 1303-6130
pISSN - 1300-0527
DOI - 10.3906/kim-2104-13
Subject(s) - chemistry , zeta potential , staphylococcus aureus , ursolic acid , chitosan , minimum inhibitory concentration , nuclear chemistry , nanoparticle , antibacterial activity , biofilm , microbiology and biotechnology , bacteria , nanotechnology , in vitro , chromatography , organic chemistry , biochemistry , materials science , biology , genetics
The present study aimed to synthesize ursolic acid-loaded chitosan nanoparticles (UA-Ch-NPs) as an antiinfective agent against 21 Staphylococcus aureus isolates. The UA-Ch-NPs were synthesized by a simple method and then characterized by TEM, FTIR, DLS-zeta potential, and XRD analyses. According to the characterization results, highly dispersed spherical nanoparticles with a mean diameter of 258 nm and a zeta potential of + 40.1 mV were developed. The antibacterial properties of UA-Ch-NPs were investigated and their inhibitory effect on biofilm formation was demonstrated by AFM. Finally, the expression levels of icaA and icaD were measured using real-time PCR. Results indicated that the minimum inhibitory concentration (MIC) of UA and UA-Ch-NPs against S. aureus was 64 and 32 µg/mL, respectively. The treatment of bacterial cells with UA-Ch-NPs significantly decreased the expression of icaA and icaD genes which are engaged in biofilm formation. Our results indicated that UA-Ch-NPs could be a promising material for antibacterial and antibiofilm applications.