Open AccessSARS-CoV-2 isolation and propagation from Turkish COVID-19 patients
Author(s) -
Cihan Taştan,
Bulut Yurtsever,
Gözde Sır Karakuş,
Derya Dilek Kançağı,
Sevda Demir,
Selen Abanuz,
Utku Seyis,
Mülazim Yildirim,
Recai Kuzay,
Ömer Elibol,
Serap Arbak,
Merve Açıkel Elmas,
Selçuk Birdoğan,
Osman Uğur Sezerman,
Ayşe Sesin Kocagöz,
Koray Yalçın,
Ercüment Ovalı
Publication year - 2020
Publication title -
turkish journal of biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.323
H-Index - 38
eISSN - 1303-6092
pISSN - 1300-0152
DOI - 10.3906/biy-2004-113
Subject(s) - outbreak , virology , isolation (microbiology) , pandemic , coronavirus , pneumonia , virus , covid-19 , biology , betacoronavirus , viral pneumonia , middle east respiratory syndrome coronavirus , disease , medicine , infectious disease (medical specialty) , microbiology and biotechnology
The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.