Open AccessA magnetic affinity approach to identify plant GABA-binding proteins
Author(s) -
Jie Zou,
Jingzhe Guo,
Shisheng Li
Publication year - 2019
Publication title -
turkish journal of biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.323
H-Index - 38
eISSN - 1303-6092
pISSN - 1300-0152
DOI - 10.3906/biy-1901-79
Subject(s) - covalent bond , biology , gabaa receptor , receptor , biochemistry , microbiology and biotechnology , chemistry , organic chemistry
In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.