Prexasertib increases the sensitivity of pancreatic cancer cells to gemcitabine and S‑1 | Zendy

Yoshihito Morimoto | Zendy; Kimihiko Takada | Zendy; Osamu Takeuchi | Zendy; Akinori Takagi | Zendy; Kazuhiro Watanabe | Zendy; Masayoshi Hirohara | Zendy; Tomoyuki Hamamoto | Zendy; Yutaka Masuda | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Prexasertib increases the sensitivity of pancreatic cancer cells to gemcitabine and S‑1

Author(s) -

Yoshihito Morimoto,

Kimihiko Takada,

Osamu Takeuchi,

Akinori Takagi,

Kazuhiro Watanabe,

Masayoshi Hirohara,

Tomoyuki Hamamoto,

Yutaka Masuda

Publication year - 2019

Publication title -

oncology reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.094

H-Index - 96

eISSN - 1791-2431

pISSN - 1021-335X

DOI - 10.3892/or.2019.7421

Subject(s) - pancreatic cancer , gemcitabine , apoptosis , chek1 , cancer research , gene knockdown , oncogene , mtt assay , cancer , cell cycle , small interfering rna , cell cycle checkpoint , cancer cell , biology , cell culture , transfection , biochemistry , genetics

Our previous study demonstrated that gemcitabine (GEM), S‑1, and a combination of GEM and S‑1 (GS) induced S‑phase arrest and increased the phosphorylation of checkpoint kinase 1 (Chk1), which is a critical mediator of cell survival under impaired DNA replication, in pancreatic cancer cell lines. The aim of the present study was to investigate the combined effect of the Chk1 inhibitor prexasertib and antitumor drugs (GEM and S‑1) on pancreatic cancer cell line SUIT‑2. Furthermore, we conducted mechanistic analysis of the combined effect. The MTT assay revealed that a combination of prexasertib and GS showed a strong effect. Mechanistic analysis of the combined effect showed effective induction of apoptosis. Furthermore, a combination of prexasertib and GS downregulated the expression of antiapoptotic protein Bcl‑2. Chk1 knockdown with small interfering RNA and GS treatment resulted in strong induction of apoptosis. Our results provide evidence to show that the combination of prexasertib and GS has a strong antitumor effect and effectively induces apoptosis in pancreatic cancer cells through downregulation of the antiapoptotic protein Bcl‑2. Prexasertib could possibly enhance the effects of standard drugs, including GEM, S‑1, and GS, against pancreatic cancer.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research