Open AccessClinicopathological and prognostic significance of microRNA-107 and its relationship to DICER1 mRNA expression in gastric cancer
Author(s) -
Hisae Iinuma
Publication year - 2012
Publication title -
oncology reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.094
H-Index - 96
eISSN - 1791-2431
pISSN - 1021-335X
DOI - 10.3892/or.2012.1709
Subject(s) - oncogene , microrna , cancer , molecular medicine , biology , stage (stratigraphy) , taqman , metastasis , cancer research , real time polymerase chain reaction , cell cycle , oncology , lymph node , pathology , medicine , gene , immunology , paleontology , biochemistry
microRNAs (miRNAs) are small non-coding RNAs that regulate target gene expression.It is known that miRNA-107 (miR-107) promotes cancer invasion and metastasis.However, the relationship between clinicopathological factors and the prognosticsignificance of miR-107 for gastric cancer patients remains elusive. In this study,we evaluated the prognostic value of miR-107 using tissue samples from gastriccancer patients. Furthermore, the relationship between miR-107 and the mRNA levelsof its target gene DICER1 was examined. The expression levels of miR-107 and DICER1mRNA in tumor tissues and adjacent normal tissues of 161 gastric cancer patientswere examined (TNM stage I, 29 patients; stage II, 31 patients; stage III, 51patients and stage IV, 50 patients). miR-107 levels were measured by Taqman microRNAassays, and DICER1 mRNA levels were measured by the Taqman real-time RT-PCR method.In the analysis by real-time PCR-based miRNA arrays using pooled RNA samples fromfive gastric cancer patients, expression of miR-107, miR-21, miR-196a, miR-26b,miR-9, miR-142-3p, miR-30b, miR-150, miR-191 and miR-17 was found to be upregulation.The mean expression level of miR-107 was significantly higher in the tumor tissuescompared to that of normal tissues. In the comparison of clinicopathological factors,miR-107 expression showed significant association with depth of tumor invasion,lymph node metastasis and stage. In Kaplan-Meier survival curve analysis, overallsurvival rates (OS) and disease-free survival rates (DFS) of patients with highmiR-107 expression were significantly worse than those of patients with low miR-107expression. In the Cox multivariate analysis, it was shown that miR-107 expressionin gastric cancer tissues was an independent prognostic factor for OS and DFS.Significant inverse correlations were demonstrated between miR-107 and DICER1mRNA. Our results indicate that miR-107 may be useful as an effective biomarkerfor prediction of a poor prognosis in gastric cancer patients.