Expression and function of miR-27b in human glioma | Zendy

Lingchao Chen | Zendy; Huibing Li | Zendy; Lei Han | Zendy; Kailiang Zhang | Zendy; Guangxiu Wang | Zendy; Yongzhi Wang | Zendy; Yanwei Liu | Zendy; Yongri Zheng | Zendy; Tao Jiang | Zendy; Peiyu Pu | Zendy; Chuanlu Jiang | Zendy; Chunsheng Kang | Zendy

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Expression and function of miR-27b in human glioma

Author(s) -

Lingchao Chen,

Huibing Li,

Lei Han,

Kailiang Zhang,

Guangxiu Wang,

Yongzhi Wang,

Yanwei Liu,

Yongri Zheng,

Tao Jiang,

Peiyu Pu,

Chuanlu Jiang,

Chunsheng Kang

Publication year - 2011

Publication title -

oncology reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.094

H-Index - 96

eISSN - 1791-2431

pISSN - 1021-335X

DOI - 10.3892/or.2011.1458

Subject(s) - glioma , oncogene , cell cycle , cancer research , microrna , apoptosis , cyclin d1 , western blot , molecular medicine , luciferase , biology , stat3 , cell growth , microbiology and biotechnology , chemistry , transfection , cell culture , gene , biochemistry , genetics

Our previous miRNAs profiling study showed that miR-27b was up-regulatedin glioma cells compared with H4 low grade astrocytoma cells. However, the mainfunction of miR-27b in glioma in not known yet. The aim of this study was to investigatethe expression and function of miR-27b in the pathogenesis of glioma. Real-timePCR showed that miR-27b was up-regulated in glioma samples and glioma cells. Down-regulationof miR-27b triggered growth inhibition, induced apoptosis and inhibited invasionin glioma cells. Furthermore, TOPflash luciferase activity was decreased significantly,while FOPflash luciferase did not change significantly. In addition, Western blotassay showed that STAT3, c-myc and cyclin D1 were knocked down after treatmentwith miR-27b inhibitor. These findings suggest that aberrantly up-regulated miR-27bmay be one of the critical factors that contribute to malignancy in human gliomas.

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