Open AccessEffects of NOTCH1 signaling inhibitor γ‑secretase inhibitor II on growth of cancer stem cells
Author(s) -
Xiaodong Ding,
Chang-qing Ding,
Fei Wang,
Wenshuai Deng,
Mingming Yu,
Qingcai Meng,
Peng Sun
Publication year - 2018
Publication title -
oncology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.766
H-Index - 54
eISSN - 1792-1082
pISSN - 1792-1074
DOI - 10.3892/ol.2018.9377
Subject(s) - oncogene , cell cycle , molecular medicine , notch signaling pathway , cancer research , cancer , cancer stem cell , cancer cell , chemistry , microbiology and biotechnology , stem cell , biology , signal transduction , genetics
The present study aimed to observe the effect of the Notch1 signaling inhibitor γ-secretase inhibitor II (GSI II) on the growth and differentiation of tumor cells. The tumor cell line U87 was grown in serum-free media, and cell growth was evaluated using immunofluorescence. Single-cell wall-adherent growing conditions were prepared, GSI II was added, and the differentiation and growth of single tumor cells was evaluated. Immunofluorescence demonstrated positive results for the expression of Nestin and cluster of differentiation 133. The cell proliferation rate was reduced following the addition of GSI II (P<0.05). GSI II may significantly inhibit the proliferation and differentiation of U87 tumor stem cells.