Open AccessA radioresistant fraction of acute promyelocytic leukemia cells exhibit CD38 cell-surface antigen and mRNA expression
Author(s) -
Satoru Monzen,
Mitsuru Chiba,
Takayoshi Ueno,
Yuki Morino,
Kenji Terada,
Hiroki Yamaya,
Yoichiro Hosokawa
Publication year - 2018
Publication title -
oncology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.766
H-Index - 54
eISSN - 1792-1082
pISSN - 1792-1074
DOI - 10.3892/ol.2018.8099
Subject(s) - radioresistance , cell cycle , acute promyelocytic leukemia , cell , oncogene , promyelocytic leukemia protein , cd38 , messenger rna , molecular medicine , antigen , leukemia , cancer research , chemistry , microbiology and biotechnology , biology , immunology , cell culture , gene , biochemistry , stem cell , genetics , cd34 , retinoic acid
In the present study, the cell viability and cluster of differentiation (CD)38 mRNA expression were evaluated in radioresistant (Res)-HL60 acute promyelocytic leukemia (APL) cells. Cell viability in Res-HL60 cells was higher compared with wild-type HL60 cells, but did not differ between high and mid/low CD38 antigen expression groups in Res-HL60 cells. A higher expression of CD38 mRNA in Res-HL60 cells was observed, particularly in the CD38 high cell subpopulation. Furthermore, the expression of CD38 mRNA was upregulated following exposure to X-irradiation. In contrast, the characteristic expression of CD45 and CCAAT/enhancer-binding protein α mRNA were not altered. These results suggest that the accumulation of CD38 protein in radioresistant APL cells, resulting from the constant expression of CD38 mRNA induced by X-irradiation, is a characteristic response of the radioresistant-surviving fraction; however, the accumulation of CD38 did not influence the extent of radioresistant behavior.