Open AccessEffect of CIK on multidrug-resistance reversal and increasing the sensitivity of ADR in K562/ADR cells
Author(s) -
Lei Wang,
Qi Deng,
Jian Wang,
Xue Bai,
Xia Xiao,
Hairong Lv,
Mingfeng Zhao,
Pengjiang Liu
Publication year - 2014
Publication title -
oncology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.766
H-Index - 54
eISSN - 1792-1082
pISSN - 1792-1074
DOI - 10.3892/ol.2014.2337
Subject(s) - survivin , k562 cells , multiple drug resistance , peripheral blood mononuclear cell , pharmacology , cancer research , p glycoprotein , apoptosis , cytokine induced killer cell , biology , medicine , immunology , leukemia , drug resistance , in vitro , biochemistry , cd8 , cd3 , antigen , microbiology and biotechnology
Leukemia is a leading cause of cancer-related mortality in children worldwide, and multidrug-resistance (MDR) is a main reason for tumor chemotherapy failure. The present study investigated the effects of ADR following incubation with cytokine-induced killer (CIK) cells on reversing MDR in K562/ADR cells. Mononuclear cells were isolated from the peripheral blood of healthy individuals and cultured in vitro in the presence of a combination of cytokines to generate CIK for K562/ADR cell treatment. A decreased level of P-glycoprotein expression and glutathione (GSH), an increased intracellular Rh-123 content, decreased mRNA and protein expression levels of MDR gene 1, MDR-associated protein 1, GSH S-transferase-π, B-cell lymphoma 2 and Survivin, and the decreased phosphorylation of AKT and the transcriptional activity of nuclear factor-κB and activator protein 1 were detected following ADR treatment in CIK co-cultured K562/ADR cells. Additionally, the level of ADR sensitivity and the apoptosis rate were increased in the CIK co-cultured K562/ADR cells. These results indicate that pre-treatment with CIK could reverse the MDR of K562/ADR cells, and that patients would be most likely to benefit from the combination of chemotherapy and CIK therapy.