Open AccessSmall compound 6-O-angeloylplenolin induces caspase-dependent apoptosis in human multiple myeloma cells
Author(s) -
Ying Li,
Ying Dong,
Bo Zhang,
YongXian Cheng
Publication year - 2013
Publication title -
oncology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.766
H-Index - 54
eISSN - 1792-1082
pISSN - 1792-1074
DOI - 10.3892/ol.2013.1370
Subject(s) - apoptosis , poly adp ribose polymerase , cell cycle , microbiology and biotechnology , cell culture , chemistry , cleavage (geology) , caspase 3 , caspase , cyclin b1 , cancer research , cyclin dependent kinase 1 , biology , programmed cell death , enzyme , polymerase , biochemistry , genetics , paleontology , fracture (geology)
6- O -angeloylplenolin (6-OAP) is a sesquiterpene lactone agent that has been previously demonstrated to inhibit the growth of multiple myeloma (MM) cells through mitotic arrest with accumulated cyclin B1. In the present study, the levels of apoptosis were analyzed in dexamethasone-sensitive (MM.1S), dexamethasone-resistant (U266) and chemotherapy-sensitive (RPMI 8226) myeloma cell lines. Enhanced apoptosis was identified following a 48-h incubation with 6-OAP (0-10 μ M) that induced a dose-dependent decrease in pro-casp-3 and the cleavage of its substrate, anti-poly (ADP-ribose) polymerase (PARP). In addition, time-dependent cleavage of PARP was also detected in U266 and MM.1S cells. The mechanism of 6-OAP cytotoxicity in all cell lines was associated with the induction of apoptosis with the presence of cleaved caspase-3 and PARP. In conclusion, 6-OAP-induced apoptosis is caspase-dependent. These observations are likely to provide a framework for future studies of 6-OAP therapy in MM.