Open AccessAssessing the protective effects of cryptotanshinone on CoCl2‑induced hypoxia in RPE cells
Author(s) -
Yuxuan Gu,
Weiming Liu,
Gaoqin Liu,
Xin Li,
Peirong Lu
Publication year - 2021
Publication title -
molecular medicine reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.727
H-Index - 56
eISSN - 1791-3004
pISSN - 1791-2997
DOI - 10.3892/mmr.2021.12379
Subject(s) - salvia miltiorrhiza , apoptosis , hypoxia (environmental) , cell cycle , microbiology and biotechnology , annexin , chemistry , retinal , cancer research , biology , pathology , medicine , biochemistry , traditional chinese medicine , oxygen , alternative medicine , organic chemistry
The development of several retinal diseases is closely related to hypoxia. As a component of the Traditional Chinese medicine Salvia miltiorrhiza , the effects of cryptotanshinone (CT) on retinal cells under hypoxic conditions are not well understood. The aim of the present study was to explore how CT exerted its protective effects on retinal pigment epithelium (RPE) cells under hypoxic conditions induced by cobalt chloride (CoCl 2 ). The effects of CT were investigated using a Cell Counting Kit‑8 assay, Annexin V‑FITC/PI staining, reverse transcription‑quantitative PCR and western blotting in ARPE‑19 cells. CT (10 and 20 µM) reduced the CoCl 2 ‑induced increase in vascular endothelial growth factor expression and hypoxia‑inducible transcription factor‑1α expression in ARPE‑19 cells. Additionally, CT alleviated hypoxia‑induced apoptosis by regulating Bcl‑2 and Bax protein expression. CT treatment also reduced the increase in the mRNA levels of IL‑6, IL‑1β and TNF‑α induced by CoCl 2 . In summary, CT may protect RPE cells against apoptosis and inflammation in CoCl 2 ‑induced hypoxia, and these results warrant further in vivo study into its value as a drug for treating hypoxic eye diseases.