Open AccessSilencing of perilipin by short hairpin RNA inhibits proliferation and induces apoptosis in liposarcoma cells
Author(s) -
L. Meng,
Yu Zheng,
MaoLei He,
Xiaoming Zhou,
Shaomei Sun,
Zhaojun Ding,
Qin Meng,
Bingcheng Li,
Yanwei Sun
Publication year - 2018
Publication title -
molecular medicine reports
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.727
H-Index - 56
eISSN - 1791-3004
pISSN - 1791-2997
DOI - 10.3892/mmr.2018.9461
Subject(s) - cell cycle , gene silencing , apoptosis , biology , cell growth , liposarcoma , cancer research , microbiology and biotechnology , flow cytometry , small hairpin rna , cell , perilipin , pathology , adipose tissue , medicine , endocrinology , gene knockdown , genetics , gene , sarcoma , lipolysis
Previous studies have identified that perilipin-1 (PLIN1) is a highly specific marker for liposarcoma. However, its functions have yet to be fully elucidated. The aim of the present study was to investigate the potential role of PLIN1 in the proliferation, migration and apoptosis of liposarcoma cells. Short hairpin RNA was designed to inhibit PLIN1 levels. Cell proliferation was monitored by Cell Counting Kit‑8 assay and cell migration determined by wound healing assay. Flow cytometry was performed to assess the cell cycle distributions and apoptosis in liposarcoma cells. The results demonstrated that the expression of PLIN1 was significantly upregulated in liposarcoma tumor tissues compared with normal adipose tissues. Silencing of PLIN1 by short hairpin RNA significantly inhibited proliferation and migration and induced G1 phase cell cycle arrest and apoptosis in liposarcoma cell lines. It was identified that PLIN1 serves a crucial role in the pathogenesis and progression of liposarcoma and may be a potential therapeutic target for its clinical management.